Rat PIIINP (N-Terminal Procollagen III Propeptide) CLIA Kit
The Rat PIIINP (N-terminal procollagen III propeptide) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of PIIINP levels in rat samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.PIIINP is an important marker for collagen synthesis and tissue remodeling, playing a key role in conditions such as liver fibrosis, renal fibrosis, and bone metabolism.
By measuring PIIINP levels, researchers can gain valuable insights into these processes and potential therapeutic targets.Overall, the Rat PIIINP CLIA Kit is a valuable tool for studying collagen metabolism and fibrotic diseases in rat models, offering precise and accurate measurements for comprehensive research studies.
Product Name:
Rat PIIINP (N-Terminal Procollagen III Propeptide) CLIA Kit
SKU:
RTES00413
Target:
Rat PIIINP (N-Terminal Procollagen III Propeptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Pâ…¢NP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Pâ…¢NP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Pâ…¢NP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Pâ…¢NP. You can calculate the concentration of Rat Pâ…¢NP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
103-115
90-104
Average (%)
99
109
96
1:4
Range (%)
93-108
93-109
97-108
Average (%)
99
99
103
1:8
Range (%)
97-109
88-103
84-100
Average (%)
103
96
91
1:16
Range (%)
92-108
98-112
95-108
Average (%)
99
105
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
100
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
92-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
210.92
353.07
1705.22
199.29
374.86
1549.84
Standard deviation
21.11
27.29
143.24
25.41
28.79
106.01
C V (%)
10.01
7.73
8.4
12.75
7.68
6.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Pâ…¢NP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Pâ…¢NP in samples. No significant cross-reactivity or interference between Rat Pâ…¢NP and analogues was observed.
