The Rat PK2 (Prokineticin 2) CLIA Kit is a powerful tool designed for the accurate and efficient detection of prokineticin 2 levels in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an indispensable asset for researchers in various fields.Prokineticin 2 is a key protein that plays a crucial role in various physiological processes, including regulating circadian rhythm, neurogenesis, and pain perception.
Dysregulation of prokineticin 2 has been linked to several diseases, such as neurological disorders and inflammatory conditions, making it an important biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Rat PK2 CLIA Kit offers a comprehensive solution for researchers seeking to investigate prokineticin 2 levels and gain insights into its biological functions and implications in health and disease.
Product Name:
Rat PK2 (Prokineticin 2) CLIA Kit
SKU:
RTES00470
Target:
Rat PK2 (Prokineticin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PK2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PK2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PK2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PK2. You can calculate the concentration of Rat PK2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
97-112
86-99
Average (%)
106
105
92
1:4
Range (%)
95-108
91-106
85-98
Average (%)
101
97
92
1:8
Range (%)
93-105
97-113
92-105
Average (%)
98
105
100
1:16
Range (%)
96-111
91-104
104-117
Average (%)
104
99
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
84-97
90
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
192.15
624.95
1944.66
205.01
663.06
2018.56
Standard deviation
16.06
51.68
197.77
25.95
55.7
123.13
C V (%)
8.36
8.27
10.17
12.66
8.4
6.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PK2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PK2 in samples. No significant cross-reactivity or interference between Rat PK2 and analogues was observed.
