Rat PPAR-gamma (Peroxisome Proliferator-Activated Receptor gamma) CLIA Kit
The Rat PPAR Gamma (Peroxisome Proliferator-Activated Receptor Gamma) CLIA Kit is a reliable and accurate tool for measuring levels of PPAR Gamma in rat samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for research purposes.PPAR Gamma is a key transcription factor that regulates various biological processes, including adipogenesis, glucose metabolism, and inflammatory responses. Dysregulation of PPAR Gamma has been linked to metabolic disorders such as obesity, diabetes, and cardiovascular diseases.
Therefore, accurate measurement of PPAR Gamma levels is crucial for studying these conditions and exploring potential therapeutic interventions.With its user-friendly design and robust performance, the Rat PPAR Gamma CLIA Kit is a valuable resource for researchers investigating the role of PPAR Gamma in health and disease. Trust Assay Genie's expertise and technology to uncover new insights into PPAR Gamma biology and its implications in metabolic disorders.
Product Name:
Rat PPAR-gamma (Peroxisome Proliferator-Activated Receptor gamma) CLIA Kit
SKU:
RTES00433
Target:
Rat PPAR-gamma (Peroxisome Proliferator-Activated Receptor gamma)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PPAR-γ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PPAR-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PPAR-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PPAR-γ. You can calculate the concentration of Rat PPAR-γ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
94-104
94-105
Average (%)
103
99
99
1:4
Range (%)
93-107
99-113
91-107
Average (%)
100
104
98
1:8
Range (%)
86-99
86-97
87-101
Average (%)
91
92
93
1:16
Range (%)
89-99
101-117
100-111
Average (%)
94
107
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-117
107
EDTA plasma (n=5)
85-99
91
Cell culture media (n=5)
100-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.9
173.22
673.93
111.84
173.93
655.93
Standard deviation
12.31
12.89
62.81
14.33
17.08
48.28
C V (%)
12.08
7.44
9.32
12.81
9.82
7.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PPAR-γ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PPAR-γ in samples. No significant cross-reactivity or interference between Rat PPAR-γ and analogues was observed.
