Rat PPAR-gamma (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
The Rat PPAR-γ (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit is a specialized assay designed to quantitatively measure the levels of Peroxisome Proliferator Activated Receptor Gamma in a variety of rat biological samples. PPAR-γ is a key nuclear receptor involved in regulating various cellular functions, including lipid metabolism, glucose homeostasis, and inflammation. It plays a critical role in mediating gene expression and maintaining metabolic balance in response to different physiological and environmental cues. Accurate quantification of PPAR-γ levels using this ELISA kit enables researchers to gain valuable insights into the molecular mechanisms underlying metabolic regulation, adipogenesis, and inflammatory pathways mediated by PPAR-γ.
Understanding the role of PPAR-γ in modulating lipid and glucose metabolism is essential for elucidating its impact on metabolic disorders such as obesity, diabetes, and cardiovascular diseases. The Rat PPAR-γ ELISA Kit offered by Assay Genie ensures exceptional sensitivity and specificity, resulting in reproducible and reliable results. Manufactured under strict quality control measures, this kit delivers robust performance, making it an ideal tool for researchers investigating the role of PPAR-γ in metabolic regulation and disease pathogenesis. Its user-friendly design further enhances its suitability for cutting-edge research in the fields of metabolism, energy homeostasis, and metabolic disease mechanisms.
Product Name:
Rat PPAR-gamma (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
SKU:
AEES00505
Target:
Rat PPAR-γ (Peroxisome Proliferator Activated Receptor Gamma)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PPAR-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PPAR-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PPAR-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PPAR-γ. You can calculate the concentration of Rat PPAR-γ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
85-99
91-106
Average (%)
92
92
99
1:4
Range (%)
88-101
82-93
82-94
Average (%)
93
88
88
1:8
Range (%)
89-103
84-96
86-99
Average (%)
95
89
93
1:16
Range (%)
86-99
85-98
86-98
Average (%)
92
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
92-107
100
Cell culture media (n=5)
85-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.57
3.92
0.51
1.51
4.22
Standard deviation
0.03
0.07
0.21
0.03
0.09
0.23
C V (%)
5.88
4.46
5.36
5.88
5.96
5.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PPAR-γ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PPAR-γ in samples. No significant cross-reactivity or interference between Rat PPAR-γ and analogues was observed.
