The Rat PR3 (Proteinase 3) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of PR3 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.PR3 (Proteinase 3) is an important enzyme involved in immune responses and inflammatory processes. It plays a key role in conditions such as autoimmune diseases, vasculitis, and other inflammatory disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.
Overall, the Rat PR3 CLIA Kit offers researchers a powerful tool for detecting and quantifying PR3 levels in rat samples, providing valuable insight into the mechanisms of immune responses and inflammatory diseases.
Product Name:
Rat PR3 (Proteinase 3) CLIA Kit
SKU:
RTES00489
Target:
Rat PR3 (Proteinase 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PR3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PR3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PR3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PR3. You can calculate the concentration of Rat PR3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
97-115
98-111
Average (%)
103
105
104
1:4
Range (%)
100-115
91-107
93-105
Average (%)
107
98
100
1:8
Range (%)
98-110
103-117
100-117
Average (%)
104
110
108
1:16
Range (%)
94-108
97-110
96-112
Average (%)
100
103
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
94-110
102
Cell culture media (n=5)
100-117
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.28
127.52
271.92
40.49
120.33
272.76
Standard deviation
3.28
13.36
23.36
3.59
8.64
22.99
C V (%)
8.14
10.48
8.59
8.87
7.18
8.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PR3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PR3 in samples. No significant cross-reactivity or interference between Rat PR3 and analogues was observed.
