The Rat PRL (Prolactin) CLIA Kit is specifically formulated for the precise measurement of prolactin levels in rat serum and plasma samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Prolactin is a hormone that plays a critical role in numerous physiological processes, including reproduction, lactation, and immune regulation. Dysregulation of prolactin levels has been associated with various health conditions, such as infertility, autoimmune disorders, and metabolic disorders.
Therefore, the Rat PRL CLIA Kit is a valuable tool for studying these conditions and exploring potential therapeutic interventions.With its user-friendly design and reliable performance, the Rat PRL CLIA Kit is an essential resource for researchers in the fields of endocrinology, reproductive biology, and immunology. Trust in this kit to deliver precise measurements and valuable insights into the role of prolactin in health and disease.
Product Name:
Rat PRL (Prolactin) CLIA Kit
SKU:
RTES00031
Target:
Rat PRL (Prolactin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PRL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PRL. You can calculate the concentration of Rat PRL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
103-118
103-118
Average (%)
96
110
110
1:4
Range (%)
97-114
96-108
93-108
Average (%)
104
102
101
1:8
Range (%)
101-120
97-111
101-117
Average (%)
110
104
109
1:16
Range (%)
88-102
91-103
86-100
Average (%)
96
96
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-104
99
EDTA plasma (n=5)
99-112
105
Cell culture media (n=5)
99-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.12
4.91
0.49
1.2
5.29
Standard deviation
0.05
0.09
0.45
0.05
0.14
0.5
C V (%)
10.2
8.04
9.16
10.2
11.67
9.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PRL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PRL in samples. No significant cross-reactivity or interference between Rat PRL and analogues was observed.
