The Rat PROC (Protein C) CLIA Kit is a specialized assay designed for the precise measurement of protein C levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, delivering accurate and consistent results for various research experiments.Protein C is a key protein involved in the regulation of blood clotting and inflammation, playing a critical role in maintaining vascular health. Dysregulation of protein C levels has been linked to various pathological conditions, including thrombosis, sepsis, and inflammatory disorders, underscoring its importance as a biomarker for disease research and therapeutic development.
With its reliable performance and ease of use, the Rat PROC (Protein C) CLIA Kit is an invaluable tool for investigating the role of protein C in different disease models and exploring potential treatment strategies. Trust Assay Genie for high-quality research tools that drive scientific discovery.
Product Name:
Rat PROC (Protein C) CLIA Kit
SKU:
RTES00483
Target:
Rat PROC (Protein C)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PROC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PROC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PROC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PROC. You can calculate the concentration of Rat PROC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-115
97-115
99-113
Average (%)
109
105
105
1:4
Range (%)
100-115
86-102
93-104
Average (%)
108
93
99
1:8
Range (%)
95-112
94-110
87-99
Average (%)
102
101
92
1:16
Range (%)
97-113
92-108
88-101
Average (%)
104
99
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
90-101
96
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.11
2.24
9.7
1.18
2.05
9.23
Standard deviation
0.13
0.16
0.78
0.14
0.23
0.75
C V (%)
11.71
7.14
8.04
11.86
11.22
8.13
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PROC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PROC in samples. No significant cross-reactivity or interference between Rat PROC and analogues was observed.
