Rat Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit
The Rat Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit is specifically designed to accurately measure levels of PTGS2 in rat samples such as serum, plasma, and tissue lysates. This kit is known for its high sensitivity and specificity, providing precise and consistent results for various research applications. PTGS2, also known as Cyclooxygenase-2 (COX-2), is an enzyme involved in the production of prostaglandins, which play a crucial role in inflammation, pain, and various disease processes. Dysregulation of PTGS2 has been linked to conditions such as inflammation, cancer, and cardiovascular disorders, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
With the Rat PTGS2 ELISA Kit, researchers can accurately quantify PTGS2 levels in rat samples, aiding in the understanding of its role in various physiological and pathological processes. Its reliable performance and ease of use make it a valuable tool for investigating the mechanisms and potential treatments related to PTGS2-related diseases.
Product Name:
Rat Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
