The Rat pTau (Phospho-Tau) Protein CLIA Kit is a cutting-edge assay kit designed for the quantitative measurement of phospho-tau protein levels in rat samples, including serum and tissue lysates. This kit offers high sensitivity and specificity, providing accurate and reliable results for researchers studying neurodegenerative disorders and Alzheimer's disease.Phospho-tau protein is a key player in the pathogenesis of neurodegenerative diseases, particularly Alzheimer's disease, where abnormal tau phosphorylation leads to the formation of neurofibrillary tangles.
By measuring phospho-tau levels in rat models, researchers can gain valuable insights into disease progression and potential therapeutic interventions.Overall, the Rat pTau Protein CLIA Kit is a valuable tool for researchers looking to explore the role of phospho-tau in neurodegenerative disorders and advance our understanding of these complex conditions.
Product Name:
Rat ptau (phospho Tau Protein) CLIA Kit
SKU:
RTES00602
Target:
Rat ptau (phospho Tau Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat pτ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat pτ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat pτ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat pτ. You can calculate the concentration of Rat pτ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
93-105
98-110
Average (%)
97
99
104
1:4
Range (%)
93-106
103-119
89-104
Average (%)
98
109
96
1:8
Range (%)
85-97
85-98
92-106
Average (%)
91
92
100
1:16
Range (%)
96-114
87-100
94-108
Average (%)
104
92
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
104
EDTA plasma (n=5)
87-98
93
Cell culture media (n=5)
101-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.58
57.76
202.79
23.42
58.72
188.34
Standard deviation
3.26
5.18
15.92
2.62
6.68
13.18
C V (%)
12.74
8.97
7.85
11.19
11.38
7.0
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat pτ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat pτ in samples. No significant cross-reactivity or interference between Rat pτ and analogues was observed.
