The Rat pTau (Phospho-Tau) Protein ELISA Kit is specifically designed for the accurate detection of phosphorylated tau protein levels in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an ideal tool for various research applications.Phosphorylated tau protein is a key player in neurodegenerative diseases such as Alzheimer's and other tauopathies.
By measuring the levels of pTau in rat samples, researchers can gain valuable insights into the progression of these diseases and potentially identify new therapeutic targets.Overall, the Rat pTau Protein ELISA Kit from Assay Genie is a valuable tool for studying neurodegenerative disorders in rat models and advancing our understanding of these complex conditions.
Product Name:
Rat ptau (phospho Tau Protein) ELISA Kit
SKU:
RTES00898
Target:
Rat ptau (phospho Tau Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat pMAPT/pTAU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat pMAPT/pTAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat pMAPT/pTAU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat pMAPT/pTAU. You can calculate the concentration of Rat pMAPT/pTAU in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
92-105
100-115
Average (%)
101
99
105
1:4
Range (%)
89-101
81-95
86-97
Average (%)
94
87
92
1:8
Range (%)
92-104
85-99
84-97
Average (%)
97
91
89
1:16
Range (%)
90-102
82-91
82-94
Average (%)
96
86
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
93
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.37
144.55
406.66
49.03
139.91
386.68
Standard deviation
2.67
7.5
14.72
3.25
7.15
13.61
C V (%)
5.1
5.19
3.62
6.63
5.11
3.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat pMAPT/pTAU concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat pMAPT/pTAU in samples. No significant cross-reactivity or interference between Rat pMAPT/pTAU and analogues was observed.
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