Rat PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) CLIA Kit
The Rat PTGS2 (COX-2) Prostaglandin-Endoperoxide Synthase-2 CLIA Kit is specifically designed for the quantitative detection of PTGS2 (COX-2) levels in rat samples. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for research purposes.PTGS2, also known as COX-2, is an enzyme involved in the production of prostaglandins, which play a crucial role in inflammatory processes. Dysregulation of PTGS2 has been linked to various diseases, including cancer, inflammation, and cardiovascular disorders, making it a valuable biomarker for studying these conditions.
By utilizing this CLIA kit, researchers can gain valuable insights into the role of PTGS2 in disease pathology and potentially identify new therapeutic targets. With its easy-to-use format and robust performance, this kit is an essential tool for researchers working in the field of inflammation and related disorders.
Product Name:
Rat PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) CLIA Kit
SKU:
RTES00479
Target:
Rat PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PTGS2/COX-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PTGS2/COX-2. You can calculate the concentration of Rat PTGS2/COX-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
96-114
101-119
Average (%)
108
104
109
1:4
Range (%)
87-100
93-104
93-106
Average (%)
93
98
100
1:8
Range (%)
101-113
97-109
90-101
Average (%)
107
103
96
1:16
Range (%)
104-118
100-115
96-111
Average (%)
110
106
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
105
EDTA plasma (n=5)
97-110
103
Cell culture media (n=5)
97-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.54
316.48
757.34
104.63
346.41
686.08
Standard deviation
8.68
33.39
83.69
11.7
26.54
48.3
C V (%)
9.09
10.55
11.05
11.18
7.66
7.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PTGS2/COX-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PTGS2/COX-2 in samples. No significant cross-reactivity or interference between Rat PTGS2/COX-2 and analogues was observed.
