Rat PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit
The Rat PTGS2 (COX-2) Prostaglandin-Endoperoxide Synthase-2 ELISA Kit is specifically designed for the precise measurement of PTGS2 levels in rat samples. This kit offers exceptional sensitivity and specificity, allowing for accurate and consistent results, making it an invaluable tool for various research purposes.PTGS2, also known as cyclooxygenase-2, is a key enzyme involved in the production of prostaglandins, which play a critical role in inflammation and pain response.
Dysregulation of PTGS2 has been implicated in various diseases, including cancer, arthritis, and cardiovascular disorders, making it a valuable biomarker for investigating these conditions and potential therapeutic interventions.With its advanced technology and reliable performance, the Rat PTGS2 (COX-2) Prostaglandin-Endoperoxide Synthase-2 ELISA Kit is a trusted tool for researchers looking to study the role of PTGS2 in health and disease in rat models.
Product Name:
Rat PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit
SKU:
RTES00660
Target:
Rat PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PTGS2/COX-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PTGS2/COX-2. You can calculate the concentration of Rat PTGS2/COX-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
91-105
96-110
Average (%)
105
99
103
1:4
Range (%)
85-99
81-93
87-100
Average (%)
92
88
93
1:8
Range (%)
88-103
88-99
81-95
Average (%)
95
93
87
1:16
Range (%)
88-100
84-97
83-95
Average (%)
94
88
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
90
EDTA plasma (n=5)
90-103
97
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
403.31
795.28
3388.82
400.56
815.3
3160.09
Standard deviation
27.43
46.92
102.34
26.44
41.17
165.59
C V (%)
6.8
5.9
3.02
6.6
5.05
5.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PTGS2/COX-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PTGS2/COX-2 in samples. No significant cross-reactivity or interference between Rat PTGS2/COX-2 and analogues was observed.
