Rat RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) CLIA Kit
The Rat RANTES (Regulated on Activation, Normal T Cell Expressed and Secreted) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of RANTES levels in rat serum, plasma, and cell culture supernatants. This kit is essential for researchers studying inflammation, immune responses, and chemotaxis, providing reliable and reproducible results for a wide range of applications.RANTES is a key chemokine involved in recruiting immune cells to sites of inflammation and infection, playing a crucial role in the regulation of immune responses.
Dysregulation of RANTES levels has been implicated in various inflammatory conditions, making it a valuable biomarker for understanding and potentially treating these diseases.Overall, the Rat RANTES CLIA Kit offers researchers a powerful tool for investigating the role of RANTES in immune responses, inflammation, and disease pathogenesis, facilitating valuable insights and potential therapeutic interventions.
Product Name:
Rat RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) CLIA Kit
SKU:
RTES00500
Target:
Rat RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat RANTES. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RANTES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RANTES, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat RANTES. You can calculate the concentration of Rat RANTES in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
90-104
91-106
Average (%)
91
97
97
1:4
Range (%)
98-111
90-102
97-108
Average (%)
105
96
102
1:8
Range (%)
88-99
99-116
90-104
Average (%)
93
106
96
1:16
Range (%)
84-96
84-96
88-102
Average (%)
91
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
91
EDTA plasma (n=5)
98-111
104
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.09
52.61
188.31
25.82
53.22
189.12
Standard deviation
2.98
5.62
16.61
2.28
4.1
16.7
C V (%)
12.37
10.68
8.82
8.83
7.7
8.83
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat RANTES concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat RANTES in samples. No significant cross-reactivity or interference between Rat RANTES and analogues was observed.
