Rat Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit
The Rat Regulated on Activation, Normal T Cell Expressed and Secreted (RANTES) ELISA Kit is specifically designed for the measurement of RANTES levels in rat samples, including serum, plasma, and tissue homogenates. With its high sensitivity and precision, this kit enables accurate and reproducible quantification of RANTES, making it an indispensable tool for researchers working in the field of immunology and inflammation.RANTES is a key chemokine involved in the regulation of immune responses, playing a critical role in recruiting and activating immune cells during inflammatory processes.
Dysregulation of RANTES has been implicated in various diseases such as autoimmune disorders, allergic reactions, and viral infections, highlighting its importance as a biomarker for studying these conditions and potential therapeutic interventions.Whether you are studying immune responses, inflammatory pathways, or exploring potential treatment options, the Rat RANTES ELISA Kit offers a reliable and efficient solution for measuring RANTES levels in rat samples with confidence and accuracy.
Product Name:
Rat Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit
Product Code:
RTDL00896
Size:
96 Assays
Target:
RANTES
Synonyms:
CCL5, SCYA5, SISd, EoCP, Chemokine C-C-Motif Ligand 5, Eosinophil Chemotactic Cytokine, T-cell Specific Protein p288, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5
Detection Method:
Sandwich
Range:
15.6-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
