Rat sCD14 (Soluble Cluster of Differentiation 14) CLIA Kit
The Rat sCD14 (Soluble Cluster of Differentiation 14) CLIA Kit is specifically designed for the quantitative measurement of soluble CD14 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional accuracy and precision, ensuring reliable and consistent results for various research applications.CD14 is a key molecule involved in the immune response, acting as a co-receptor for bacterial lipopolysaccharide (LPS) and facilitating the recognition of pathogens by the immune system.
Elevated levels of soluble CD14 have been linked to inflammatory conditions, sepsis, and other immune-related disorders, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.With its high sensitivity and specificity, the Rat sCD14 CLIA Kit provides researchers with a powerful tool for investigating the role of soluble CD14 in health and disease, ultimately advancing our understanding of immune function and pathology in rat models.
Product Name:
Rat sCD14 (Soluble Cluster of Differentiation 14) CLIA Kit
SKU:
RTES00515
Target:
Rat sCD14 (Soluble Cluster of Differentiation 14)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat sCD14. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat sCD14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat sCD14, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat sCD14. You can calculate the concentration of Rat sCD14 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
88-100
98-114
Average (%)
100
95
104
1:4
Range (%)
103-120
101-115
101-116
Average (%)
110
107
106
1:8
Range (%)
85-97
95-110
98-112
Average (%)
91
101
103
1:16
Range (%)
92-107
94-105
95-108
Average (%)
99
100
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
89-104
96
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.04
3.18
9.01
1.08
3.18
9.32
Standard deviation
0.12
0.3
0.74
0.1
0.27
0.97
C V (%)
11.54
9.43
8.21
9.26
8.49
10.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat sCD14 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat sCD14 in samples. No significant cross-reactivity or interference between Rat sCD14 and analogues was observed.
