The Rat SDC4 (Syndecan-4) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Syndecan-4 levels in rat samples. This kit provides reliable and reproducible results, making it an ideal tool for research applications in the fields of cell biology, metabolism, and inflammation.Syndecan-4 is a cell surface proteoglycan that plays a crucial role in cell adhesion, migration, and signaling. It is involved in various physiological processes, including wound healing, tissue regeneration, and inflammation.
Dysregulation of Syndecan-4 has been linked to diseases such as cancer, cardiovascular disorders, and autoimmune conditions, highlighting its importance as a biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Rat SDC4 CLIA Kit offers researchers a powerful tool for studying the function and regulation of Syndecan-4 in rat models, providing valuable insights into its role in health and disease processes.
Product Name:
Rat SDC4 (Syndecan 4) CLIA Kit
SKU:
RTES00532
Target:
Rat SDC4 (Syndecan 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SDC4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDC4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDC4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SDC4. You can calculate the concentration of Rat SDC4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
91-107
97-110
Average (%)
95
98
104
1:4
Range (%)
90-101
86-100
89-101
Average (%)
96
93
96
1:8
Range (%)
100-117
86-98
92-104
Average (%)
108
93
98
1:16
Range (%)
101-116
99-116
97-112
Average (%)
108
107
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
101
EDTA plasma (n=5)
85-97
91
Cell culture media (n=5)
86-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
210.9
545.01
1531.82
197.56
516.17
1430.71
Standard deviation
23.35
42.35
122.39
22.88
49.24
111.02
C V (%)
11.07
7.77
7.99
11.58
9.54
7.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SDC4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SDC4 in samples. No significant cross-reactivity or interference between Rat SDC4 and analogues was observed.
