The Rat SHH (Hedgehog Homolog Sonic) CLIA Kit is a state-of-the-art assay kit designed for the quantitative detection of Sonic Hedgehog Homolog levels in rat samples. This kit is optimized for use with serum, plasma, and tissue homogenates, offering high sensitivity and specificity for reliable and reproducible results.Sonic Hedgehog Homolog is a critical signaling molecule involved in embryonic development and tissue regeneration. Dysregulation of SHH signaling has been implicated in various diseases, including cancer, developmental disorders, and neurological conditions.
Therefore, the accurate measurement of SHH levels is essential for understanding disease mechanisms and developing targeted therapies.The Rat SHH CLIA Kit provides researchers with a valuable tool for studying SHH signaling in a preclinical rat model, enabling comprehensive investigations into the role of Sonic Hedgehog Homolog in disease pathogenesis and therapeutic interventions. Trust in the accuracy and precision of this kit to advance your research in developmental biology, oncology, and regenerative medicine.
Product Name:
Rat SHH (Hedgehog Homolog, Sonic) CLIA Kit
SKU:
RTES00283
Target:
Rat SHH (Hedgehog Homolog, Sonic)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SHH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SHH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SHH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SHH. You can calculate the concentration of Rat SHH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
100-116
96-111
Average (%)
93
108
103
1:4
Range (%)
90-103
85-98
94-105
Average (%)
95
91
99
1:8
Range (%)
96-110
89-99
97-115
Average (%)
102
94
105
1:16
Range (%)
88-103
103-118
89-100
Average (%)
94
109
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
94
EDTA plasma (n=5)
100-116
107
Cell culture media (n=5)
90-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.66
175.67
970.2
92.27
188.99
893.31
Standard deviation
9.36
19.34
66.36
10.85
18.16
78.52
C V (%)
9.49
11.01
6.84
11.76
9.61
8.79
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SHH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SHH in samples. No significant cross-reactivity or interference between Rat SHH and analogues was observed.
