Rat sICAM-1/CD54 (Intercellular Adhesion Molecule-1) CLIA Kit
The Rat SICAM-1 (CD54) Intercellular Adhesion Molecule-1 CLIA Kit is specifically designed for the precise measurement of SICAM-1 levels in rat biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for various research applications.SICAM-1, also known as CD54, is a key adhesion molecule involved in mediating interactions between cells, particularly in the immune response and inflammatory processes.
Its levels have been linked to various diseases and conditions, including autoimmune disorders, infections, and inflammatory diseases. Therefore, studying SICAM-1 levels can provide insights into disease mechanisms and potential therapeutic targets.Overall, the Rat SICAM-1 (CD54) Intercellular Adhesion Molecule-1 CLIA Kit is a reliable and efficient solution for researchers looking to investigate the role of SICAM-1 in the context of rat models and biological samples.
Product Name:
Rat sICAM-1/CD54 (Intercellular Adhesion Molecule-1) CLIA Kit
SKU:
RTES00028
Target:
Rat sICAM-1/CD54 (Intercellular Adhesion Molecule-1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ICAM-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ICAM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ICAM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ICAM-1. You can calculate the concentration of Rat ICAM-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
92-106
86-100
Average (%)
94
97
92
1:4
Range (%)
85-96
104-117
89-100
Average (%)
90
110
95
1:8
Range (%)
101-119
91-104
101-113
Average (%)
109
96
106
1:16
Range (%)
91-104
100-118
99-112
Average (%)
98
108
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-112
102
EDTA plasma (n=5)
90-106
98
Cell culture media (n=5)
100-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
44.25
81.34
304.78
44.72
81.8
279.6
Standard deviation
5.41
8.44
24.38
4.98
7.05
25.58
C V (%)
12.23
10.38
8.0
11.14
8.62
9.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ICAM-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ICAM-1 in samples. No significant cross-reactivity or interference between Rat ICAM-1 and analogues was observed.
