The Rat SIGA (Secretory Immunoglobulin A) CLIA Kit is a cutting-edge assay designed for the quantitative measurement of secretory immunoglobulin A levels in rat samples. This innovative kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Secretory immunoglobulin A is a key component of the body's immune defense system, particularly in mucosal surfaces such as the gut and respiratory tract.
Monitoring SIGA levels can provide valuable insights into the immune response and mucosal immunity, making this kit essential for studying immune-related diseases and therapies.With its easy-to-use format and reliable performance, the Rat SIGA CLIA Kit is a valuable tool for researchers looking to explore the role of secretory immunoglobulin A in health and disease. Order yours today and unlock new possibilities in your research endeavors.
Product Name:
Rat sIgA (Secretory Immunoglobulin A) CLIA Kit
SKU:
RTES00509
Target:
Rat sIgA (Secretory Immunoglobulin A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat sIgA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat sIgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat sIgA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat sIgA. You can calculate the concentration of Rat sIgA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
85-97
93-107
Average (%)
107
90
100
1:4
Range (%)
103-118
91-105
92-107
Average (%)
109
98
99
1:8
Range (%)
94-109
92-107
88-104
Average (%)
102
98
95
1:16
Range (%)
93-106
98-110
101-118
Average (%)
98
104
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
89-104
95
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.17
3.5
18.49
2.17
3.24
16.95
Standard deviation
0.18
0.37
1.98
0.26
0.34
1.33
C V (%)
8.29
10.57
10.71
11.98
10.49
7.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat sIgA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat sIgA in samples. No significant cross-reactivity or interference between Rat sIgA and analogues was observed.
