SynonymsSIR2L3,Silent Mating Type Information Regulation 2 Homolog 3,NAD-dependent protein deacetylase sirtuin-3, mitochondrial,SIR2-like protein 3,Regulatory protein SIR2 homolog 3
ReactivityRat
Sample TypeTissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
RTDL00957
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
B2RZ31
Sensitivity:
0.088ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SIR2L3,Silent Mating Type Information Regulation 2 Homolog 3,NAD-dependent protein deacetylase sirtuin-3, mitochondrial,SIR2-like protein 3,Regulatory protein SIR2 homolog 3
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Rat Sirtuin-3 (Sirt3) ELISA Kit is a highly sensitive and specific assay designed for the precise measurement of Sirt3 levels in rat samples, including serum, plasma, and cell culture supernatants. With its reliable and reproducible results, this kit is perfect for a variety of research applications.Sirt3 is a key protein involved in regulation of metabolism, energy production, and oxidative stress response.
It plays a critical role in age-related diseases, such as metabolic disorders, neurodegenerative diseases, and cardiovascular problems. By accurately detecting Sirt3 levels, researchers can gain valuable insights into these conditions and potentially develop new therapeutic approaches.
Product Name:
Rat Sirtuin 3 (SIRT3) ELISA Kit
Product Code:
RTDL00957
Size:
96 Assays
Target:
SIRT3
Synonyms:
SIR2L3, Silent Mating Type Information Regulation 2 Homolog 3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, SIR2-like protein 3, Regulatory protein SIR2 homolog 3
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
