Rat TARC (Thymus Activation Regulated Chemokine) CLIA Kit
The Rat TARC (Thymus Activation Regulated Chemokine) CLIA Kit is a powerful tool for measuring TARC levels in rat samples. This kit is specifically designed for accurate quantification of TARC in serum, plasma, and tissue culture supernatants, with high sensitivity and specificity to ensure reliable results.TARC is a chemokine that plays a crucial role in immune responses, particularly in regulating T cell migration and activation. Elevated levels of TARC have been associated with various inflammatory and autoimmune conditions, making it an important biomarker for research in immunology, allergy, and inflammatory diseases.
With its ease of use and robust performance, the Rat TARC CLIA Kit is an essential tool for researchers studying immune responses and inflammatory processes in rat models. Order now to accelerate your research and uncover new insights into the role of TARC in health and disease.
Product Name:
Rat TARC (Thymus Activation Regulated Chemokine) CLIA Kit
SKU:
RTES00550
Target:
Rat TARC (Thymus Activation Regulated Chemokine)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TARC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TARC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TARC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TARC. You can calculate the concentration of Rat TARC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
98-112
101-117
Average (%)
91
105
109
1:4
Range (%)
98-114
90-105
88-103
Average (%)
105
98
94
1:8
Range (%)
89-101
96-110
101-114
Average (%)
94
101
107
1:16
Range (%)
92-106
100-114
100-116
Average (%)
100
107
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
101
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
85-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.59
47.89
219.47
26.63
46.49
212.42
Standard deviation
2.89
5.21
20.32
3.28
4.66
15.36
C V (%)
10.87
10.88
9.26
12.32
10.02
7.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TARC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TARC in samples. No significant cross-reactivity or interference between Rat TARC and analogues was observed.
