Rat TDGF1 (Teratocarcinoma Derived Growth Factor 1) CLIA Kit
The Rat TDGF1 (Teratocarcinoma-Derived Growth Factor 1) CLIA Kit is specifically designed for the accurate detection of TDGF1 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for various research applications.TDGF1 is a vital growth factor that plays a crucial role in embryonic development and stem cell maintenance. It is also implicated in various physiological processes, including cell proliferation and differentiation.
Dysregulation of TDGF1 expression has been linked to conditions such as teratocarcinoma and other developmental disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Rat TDGF1 CLIA Kit provides researchers with a powerful tool for investigating the role of TDGF1 in various biological processes and disease pathways, facilitating the advancement of scientific knowledge and the development of novel treatment strategies.
Product Name:
Rat TDGF1 (Teratocarcinoma Derived Growth Factor 1) CLIA Kit
SKU:
RTES00541
Target:
Rat TDGF1 (Teratocarcinoma Derived Growth Factor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TDGF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TDGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TDGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TDGF1. You can calculate the concentration of Rat TDGF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
93-108
102-117
Average (%)
96
99
108
1:4
Range (%)
98-111
91-101
98-111
Average (%)
105
96
105
1:8
Range (%)
92-103
92-104
86-99
Average (%)
97
98
91
1:16
Range (%)
90-103
92-102
86-98
Average (%)
96
97
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
96-109
103
Cell culture media (n=5)
95-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.48
137.99
413.27
54.43
146.82
448.55
Standard deviation
5.71
11.2
25.62
5.99
16.63
46.11
C V (%)
10.29
8.12
6.2
11.0
11.33
10.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TDGF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TDGF1 in samples. No significant cross-reactivity or interference between Rat TDGF1 and analogues was observed.
