The Rat Transferrin (TF) ELISA Kit is a sophisticated assay designed to accurately quantify the levels of transferrin in a variety of biological samples derived from rat models. Transferrin, a glycoprotein crucial for iron transport and homeostasis, plays a pivotal role in various physiological processes, including iron binding, delivery, and regulation, making it a key player in iron metabolism. This ELISA kit offers researchers a reliable and precise method for assessing transferrin levels, providing valuable insights into iron transport mechanisms and iron-related disorders.
By enabling quantitative analysis of transferrin with exceptional sensitivity and specificity, researchers can deepen their understanding of iron metabolism, anemia, and other iron-related conditions in rat models. Manufactured under strict quality control standards, the Rat Transferrin ELISA Kit guarantees robust performance and accurate results, making it an indispensable tool for research studies focusing on iron homeostasis, transferrin biology, and iron-related disorders in rat models.
Product Name:
Rat TF (Transferrin) ELISA Kit
SKU:
AEES00542
Target:
Rat TF (Transferrin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TF. You can calculate the concentration of Rat TF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-109
100-110
92-102
Average (%)
104
105
99
1:4
Range (%)
98-106
95-103
84-95
Average (%)
101
98
90
1:8
Range (%)
97-109
89-101
96-111
Average (%)
102
97
104
1:16
Range (%)
87-99
88-96
86-96
Average (%)
95
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-109
105
EDTA plasma (n=5)
88-100
92
Cell culture media (n=5)
91-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
160.09
440.88
1702.97
160.83
442.91
1988.89
Standard deviation
9.61
20.28
92.13
8.91
20.46
80.95
C V (%)
6
4.6
5.41
5.54
4.62
4.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TF in samples. No significant cross-reactivity or interference between Rat TF and analogues was observed.
