The Rat TFF3 (Trefoil Factor 3) Intestinal CLIA Kit is a powerful tool for assessing levels of TFF3 in rat samples. This kit provides accurate and reliable results with high sensitivity and specificity, making it versatile for various research applications.TFF3, also known as trefoil factor 3, is a key protein found in the intestine that plays a crucial role in mucosal protection, wound healing, and maintaining gut health.
Abnormal levels of TFF3 have been linked to various gastrointestinal disorders, making it an important biomarker for studying intestinal health and disease progression.By utilizing the Rat TFF3 Intestinal CLIA Kit, researchers can gain valuable insights into the role of TFF3 in gut physiology and pathology, ultimately leading to a better understanding of intestinal diseases and the development of targeted therapies.
Product Name:
Rat TFF3 (Trefoil Factor 3, Intestinal) CLIA Kit
SKU:
RTES00573
Target:
Rat TFF3 (Trefoil Factor 3, Intestinal)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TFF3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TFF3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TFF3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TFF3. You can calculate the concentration of Rat TFF3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
90-104
103-115
Average (%)
98
96
108
1:4
Range (%)
91-103
87-98
96-113
Average (%)
97
93
104
1:8
Range (%)
91-104
91-103
100-112
Average (%)
98
97
106
1:16
Range (%)
97-113
88-101
90-102
Average (%)
103
93
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
102
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
102-117
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.09
170.94
805.19
102.77
171.48
777.5
Standard deviation
12.2
14.5
61.6
9.3
18.23
48.98
C V (%)
12.31
8.48
7.65
9.05
10.63
6.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TFF3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TFF3 in samples. No significant cross-reactivity or interference between Rat TFF3 and analogues was observed.
