The Rat TFR (Transferrin Receptor) CLIA Kit is specifically designed for the quantitative measurement of transferrin receptor levels in rat samples such as serum, plasma, and tissue lysates. This kit offers high sensitivity and accuracy, providing researchers with reliable and reproducible results for their studies.The transferrin receptor is a key protein involved in iron metabolism and cellular iron uptake. Abnormal levels of transferrin receptor expression have been associated with various diseases, including anemia, cancer, and neurodegenerative disorders.
By targeting the transferrin receptor, researchers can gain valuable insights into these conditions and potentially develop new therapeutic strategies.Overall, the Rat TFR CLIA Kit is a valuable tool for investigating the role of transferrin receptor in health and disease, providing researchers with the means to advance their understanding of cellular iron homeostasis and its implications for human health.
Product Name:
Rat TFR (Transferrin Receptor) CLIA Kit
SKU:
RTES00565
Target:
Rat TFR (Transferrin Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TFR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TFR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TFR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TFR1. You can calculate the concentration of Rat TFR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
91-103
100-112
Average (%)
109
98
106
1:4
Range (%)
96-114
100-116
98-112
Average (%)
104
108
104
1:8
Range (%)
86-99
88-99
96-111
Average (%)
93
93
103
1:16
Range (%)
94-106
91-107
100-115
Average (%)
100
97
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
107
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
213.22
641.83
1438.97
203.64
693.8
1526.12
Standard deviation
17.36
75.16
107.06
21.44
51.9
153.68
C V (%)
8.14
11.71
7.44
10.53
7.48
10.07
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TFR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TFR1 in samples. No significant cross-reactivity or interference between Rat TFR1 and analogues was observed.
