Rat TGF-beta1 (Transforming Growth Factor Beta 1) ELISA Kit
The Rat TGF-β1 (Transforming Growth Factor Beta 1) ELISA Kit is a specialized assay tool designed for the precise and quantitative measurement of TGF-β1 levels in rat biological samples. TGF-β1 is a pivotal cytokine known for its multifaceted roles in regulating cell growth, differentiation, immunity, and tissue repair. Its dysregulation has been associated with various pathological conditions, including fibrosis, cancer, and autoimmune diseases, underscoring the importance of accurate TGF-β1 quantification in elucidating disease mechanisms and therapeutic interventions. This ELISA kit enables researchers to explore the intricate functions of TGF-β1 in biological processes and diseases by providing exceptional sensitivity and specificity for reliable and reproducible results.
With stringent quality control measures and user-friendly protocols, the Rat TGF-β1 ELISA Kit ensures robust performance, making it an excellent choice for cutting-edge research in immunology, oncology, and regenerative medicine. Trust the Rat TGF-β1 ELISA Kit to advance your understanding of TGF-β1 biology and its implications in health and disease.
Product Name:
Rat TGF-beta1 (Transforming Growth Factor Beta 1) ELISA Kit
SKU:
AEES00320
Target:
Rat TGF-β1 (Transforming Growth Factor Beta 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TGF-β1. You can calculate the concentration of Rat TGF-β1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
84-98
92-109
Average (%)
104
91
100
1:4
Range (%)
99-115
86-97
96-112
Average (%)
106
92
102
1:8
Range (%)
102-113
81-93
98-111
Average (%)
107
87
104
1:16
Range (%)
100-117
83-93
96-107
Average (%)
107
88
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.2
170.7
940.7
112.6
182.5
874.9
Standard deviation
6
7.3
38.6
7.4
8.9
34.1
C V (%)
5.76
4.28
4.1
6.57
4.88
3.9
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TGF-β1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TGF-β1 in samples. No significant cross-reactivity or interference between Rat TGF-β1 and analogues was observed.
