The Rat TPA (Tissue Plasminogen Activator) CLIA Kit from Assay Genie is a reliable and accurate assay designed for the quantification of TPA levels in rat samples. This kit provides high sensitivity and specificity, allowing for precise and reproducible results in a variety of research applications.TPA is a key enzyme that plays a vital role in the breakdown of blood clots, making it essential for maintaining proper blood circulation and preventing thrombotic events. Dysregulation of TPA levels has been implicated in various cardiovascular conditions, making it a valuable marker for studying these diseases and exploring potential therapeutic interventions.
With the Rat TPA CLIA Kit from Assay Genie, researchers can confidently measure TPA levels in rat samples with ease and precision, facilitating their studies on thrombotic diseases and related research areas. Trust Assay Genie for accurate and dependable results in your TPA research.
Product Name:
Rat tPA (Plasminogen Activator, Tissue) CLIA Kit
SKU:
RTES00444
Target:
Rat tPA (Plasminogen Activator, Tissue)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat tPA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat tPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat tPA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat tPA. You can calculate the concentration of Rat tPA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
99-117
96-112
Average (%)
101
107
103
1:4
Range (%)
87-97
93-107
101-113
Average (%)
92
101
107
1:8
Range (%)
94-107
88-101
99-113
Average (%)
101
95
107
1:16
Range (%)
97-114
91-104
88-101
Average (%)
105
96
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-107
98
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
89-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
219.85
595.24
1904.91
210.82
584.31
1848.28
Standard deviation
20.47
50.3
151.06
16.91
62.05
124.2
C V (%)
9.31
8.45
7.93
8.02
10.62
6.72
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat tPA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat tPA in samples. No significant cross-reactivity or interference between Rat tPA and analogues was observed.
