The Rat TSLP (Thymic Stromal Lymphopoietin) CLIA Kit is specifically designed to accurately measure TSLP levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an invaluable tool for researchers involved in studying immune responses and inflammatory diseases in rats.TSLP is a key cytokine that plays a crucial role in regulating immune responses, particularly in the development of allergic inflammation and autoimmune diseases.
By measuring TSLP levels, researchers can gain important insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the Rat TSLP CLIA Kit offers a comprehensive solution for studying TSLP biology in rat models and advancing our understanding of immune-mediated diseases.
Product Name:
Rat TSLP (Thymic Stromal Lymphopoietin) CLIA Kit
SKU:
RTES00549
Target:
Rat TSLP (Thymic Stromal Lymphopoietin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TSLP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TSLP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TSLP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TSLP. You can calculate the concentration of Rat TSLP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
87-101
90-103
Average (%)
106
94
95
1:4
Range (%)
85-100
91-105
94-109
Average (%)
91
96
101
1:8
Range (%)
97-112
100-116
102-118
Average (%)
104
108
109
1:16
Range (%)
90-104
91-104
102-115
Average (%)
98
96
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
90-105
96
Cell culture media (n=5)
100-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.98
68.9
205.22
26.67
71.2
186.98
Standard deviation
2.01
5.64
18.82
2.69
7.65
17.89
C V (%)
8.05
8.19
9.17
10.09
10.74
9.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TSLP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TSLP in samples. No significant cross-reactivity or interference between Rat TSLP and analogues was observed.
