Rat Uncoupling Protein 2, Mitochondrial (UCP2) ELISA Kit
The Rat Uncoupling Protein 2 Mitochondrial (UCP2) ELISA Kit is a powerful tool for the precise measurement of UCP2 levels in rat samples including serum, plasma, and cell culture supernatants. This kit has been designed with exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.UCP2 is a mitochondrial protein that plays a crucial role in energy metabolism by regulating proton leak and uncoupling oxidative phosphorylation.
Dysregulation of UCP2 has been linked to various metabolic disorders and conditions such as obesity, diabetes, and neurodegenerative diseases, making it a valuable biomarker for studying these conditions and potential therapeutic targets.Overall, the Rat UCP2 ELISA Kit from AssayGenie provides researchers with a reliable and efficient method for quantifying UCP2 levels in rat samples, facilitating advances in metabolic research and potential treatment strategies.
Product Name:
Rat Uncoupling Protein 2, Mitochondrial (UCP2) ELISA Kit
Product Code:
RTDL01081
Size:
96 Assays
Target:
UCP2
Synonyms:
SLC25A8, UCPH, Solute carrier family 25 member 8
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
