Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit
The Rat c-Myc (Myelocytomatosis Viral Oncogene Homolog) ELISA Kit is a powerful tool for measuring levels of c-Myc in rat serum, plasma, and cell culture supernatants with high accuracy. With its exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it suitable for various research applications.c-Myc is a key oncogene involved in regulating cell growth, differentiation, and apoptosis. Dysregulation of c-Myc has been linked to various cancers, making it a valuable biomarker for cancer research and drug development.
By accurately measuring c-Myc levels, researchers can gain insights into cancer progression and potentially identify new therapeutic targets.Overall, the Rat c-Myc ELISA Kit offers a comprehensive solution for studying the role of c-Myc in cancer and other diseases, providing researchers with a valuable tool for advancing their understanding of oncogenesis and developing novel treatment strategies.
Product Name:
Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit
Product Code:
RTDL00703
Size:
96 Assays
Target:
MYC
Synonyms:
C-Myc, BHLHE39, Myc Proto-Oncogene Protein, Class E basic helix-loop-helix protein 39, Transcription factor p64, Proto-oncogene c-Myc
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
