Rat VEGF-C (Vascular Endothelial Cell Growth Factor C) CLIA Kit
The Rat VEGF-C (Vascular Endothelial Cell Growth Factor C) CLIA Kit (RTES00588) is specifically designed for the quantitative measurement of VEGF-C levels in rat serum, plasma, and tissue homogenates. This kit offers outstanding sensitivity and accuracy, ensuring precise and reliable results for a variety of research applications.VEGF-C is a key factor in regulating lymphangiogenesis and angiogenesis, playing a crucial role in the formation and maintenance of blood and lymphatic vessels.
Its dysregulation has been implicated in various pathological conditions, including cancer, inflammation, and lymphatic disorders. Therefore, the Rat VEGF-C CLIA Kit serves as a valuable tool for investigating the role of VEGF-C in these diseases and developing potential therapeutic interventions.
Product Name:
Rat VEGF-C (Vascular Endothelial Cell Growth Factor C) CLIA Kit
SKU:
RTES00588
Target:
Rat VEGF-C (Vascular Endothelial Cell Growth Factor C)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat VEGF-C. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VEGF-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VEGF-C, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat VEGF-C. You can calculate the concentration of Rat VEGF-C in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
87-102
84-95
Average (%)
100
94
90
1:4
Range (%)
101-118
99-115
94-109
Average (%)
108
105
100
1:8
Range (%)
92-106
92-106
87-98
Average (%)
100
99
92
1:16
Range (%)
96-109
87-101
100-112
Average (%)
101
93
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
92
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.52
104.37
276.29
43.25
105.12
290.1
Standard deviation
4.36
12.32
24.56
4.75
10.54
19.76
C V (%)
10.25
11.8
8.89
10.98
10.03
6.81
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat VEGF-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VEGF-C in samples. No significant cross-reactivity or interference between Rat VEGF-C and analogues was observed.
