The Rat VIP (Vasoactive Intestinal Peptide) ELISA Kit is a reliable tool for precise measurement of vasoactive intestinal peptide levels in rat samples, including serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reproducible results for various research applications. Vasoactive intestinal peptide is a vital neuropeptide that plays a crucial role in modulating various physiological processes, including neurotransmission, immune response, and vasodilation.
Dysregulation of VIP has been associated with various diseases, such as inflammatory disorders, neurodegenerative diseases, and gastrointestinal disorders, making it an essential biomarker for understanding disease mechanisms and potential therapeutic interventions. With the Rat VIP ELISA Kit, researchers can confidently analyze VIP levels in rat samples with precision and confidence, unlocking valuable insights into the role of VIP in health and disease.
Product Name:
Rat VIP (Vasoactive Intestinal Peptide) ELISA Kit
SKU:
RTES00882
Target:
Rat VIP (Vasoactive Intestinal Peptide)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VIP. During the reaction, VIP in the sample or standard competes with a fixed amount of VIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VIP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VIP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
92-110
96-109
Average (%)
98
100
102
1:4
Range (%)
88-100
91-106
98-109
Average (%)
93
97
104
1:8
Range (%)
88-100
94-107
94-108
Average (%)
93
99
101
1:16
Range (%)
84-97
88-102
95-106
Average (%)
89
94
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
94-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
22.7
51.5
221.7
20.8
55.0
202.0
Standard deviation
1.4
2.2
11.5
1.4
2.3
6.9
C V (%)
6.17
4.27
5.19
6.73
4.18
3.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of VIP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes VIP in samples. No significant cross-reactivity or interference between VIP and analogues was observed.
