The Rat VIP (Vasoactive Intestinal Peptide) ELISA Kit is a reliable tool for precise measurement of vasoactive intestinal peptide levels in rat samples, including serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reproducible results for various research applications. Vasoactive intestinal peptide is a vital neuropeptide that plays a crucial role in modulating various physiological processes, including neurotransmission, immune response, and vasodilation. Dysregulation of VIP has been associated with various diseases, such as inflammatory disorders, neurodegenerative diseases, and gastrointestinal disorders, making it an essential biomarker for understanding disease mechanisms and potential therapeutic interventions. With the Rat VIP ELISA Kit, researchers can confidently analyze VIP levels in rat samples with precision and confidence, unlocking valuable insights into the role of VIP in health and disease.
Product Name:
Rat VIP (Vasoactive Intestinal Peptide) ELISA Kit
SKU:
RTES00882
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
