The Rat Vitamin D Receptor (VDR) ELISA Kit is specifically designed for the precise measurement of vitamin D receptor levels in rat samples such as serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and selectivity, ensuring accurate and dependable results for diverse research purposes.The vitamin D receptor is a key protein that plays a critical role in various biological processes, including calcium homeostasis, immune function, and cell growth regulation. Dysregulation of the VDR has been linked to numerous diseases such as osteoporosis, autoimmune disorders, and certain types of cancer, highlighting its importance as a valuable biomarker for understanding disease pathogenesis and exploring therapeutic interventions.
By utilizing the Rat Vitamin D Receptor (VDR) ELISA Kit, researchers can uncover valuable insights into the role of VDR in health and disease, advancing our understanding of vitamin D biology and its implications for human and animal health. Trust in this innovative kit to deliver precise and reliable results for your research needs.
Product Name:
Rat Vitamin D Receptor (VDR) ELISA Kit
Product Code:
RTDL01097
Size:
96 Assays
Target:
VDR
Synonyms:
NR1I1, Nuclear Receptor Subfamily 1 Group I Member 1, Calcitriol Receptor, 1,25-dihydroxyvitamin D3 receptor
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
