The Rat VWF (Von Willebrand Factor) CLIA Kit is specifically designed for the precise measurement of VWF levels in rat serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring consistent and accurate results for various research applications.Von Willebrand Factor is a key protein involved in blood clotting and plays a critical role in primary hemostasis.
Dysregulation of VWF levels is associated with various bleeding disorders and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing targeted therapies.Overall, the Rat VWF CLIA Kit from Assay Genie provides researchers with a reliable tool to investigate VWF biology and its implications in disease mechanisms.
Product Name:
Rat VWF (Von Willebrand Factor) CLIA Kit
SKU:
RTES00596
Target:
Rat VWF (Von Willebrand Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat VWF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VWF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VWF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat VWF. You can calculate the concentration of Rat VWF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
97-112
101-115
Average (%)
91
103
108
1:4
Range (%)
92-105
89-103
101-115
Average (%)
97
96
109
1:8
Range (%)
103-116
91-106
94-109
Average (%)
109
97
101
1:16
Range (%)
98-112
89-104
88-100
Average (%)
104
96
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
101
EDTA plasma (n=5)
95-109
100
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.77
206.02
743.87
115.47
190.3
718.62
Standard deviation
10.84
15.39
51.48
10.38
17.2
81.13
C V (%)
10.15
7.47
6.92
8.99
9.04
11.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat VWF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VWF in samples. No significant cross-reactivity or interference between Rat VWF and analogues was observed.
