Rat Wingless Type MMTV IntegRat ion Site Family, Member 5A (WNT5A) ELISA Kit
The Rat Wingless-Type MMTV Integration Site Family Member 5A (Wnt5a) ELISA Kit is a powerful tool for the quantitative measurement of Wnt5a levels in rat samples including serum, plasma, and tissue homogenates. Wnt5a is a key signaling protein that plays a crucial role in various biological processes such as cell growth, differentiation, and development.This ELISA kit offers high sensitivity and specificity, ensuring accurate and reproducible results for your research needs.
By measuring Wnt5a levels, researchers can gain valuable insights into the role of this important protein in various physiological and pathological conditions. This kit is essential for studying Wnt5a-related pathways and developing potential therapeutic strategies for related diseases. Upgrade your research with the Rat Wnt5a ELISA Kit today.
Product Name:
Rat Wingless Type MMTV IntegRat ion Site Family, Member 5A (WNT5A) ELISA Kit
Product Code:
RTDL01118
Size:
96 Assays
Target:
WNT5A
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-99
94
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
