The RBP4 Antibody (CAB1600) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples.RBP4, also known as retinol-binding protein 4, plays a crucial role in the transport of retinol (vitamin A) in the blood and has been implicated in the development of insulin resistance and metabolic disorders. By targeting RBP4, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
RBP4 Antibody
SKU:
CAB1600
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RDCCAS, MCOPCB10, RBP4
Positive Sample:
SW620, HeLa, OVCAR3, Mouse liver, Mouse spleen
Cellular Localization:
Secreted.
Calculated MW:
21kDa
Observed MW:
23kDa
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells.
Purification Method
Affinity purification
Gene ID
5950
RRID
AB_2763440
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using RBP4 Rabbit pAb (CAB1600) at 1:300 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC) :HAP1 Exposure time: 30s.
