The RCC1 Monoclonal Antibody (CAB0662) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the RCC1 protein, this antibody facilitates the detection and analysis of RCC1 in various cell types, making it an invaluable tool for investigations in cellular biology and cancer research.RCC1, also referred to as regulator of chromosome condensation 1, is crucial for the proper regulation of the cell cycle and nuclear envelope dynamics.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RCC1 Monoclonal Antibody
SKU:
CAB0662
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1834
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CHC1, RCC1-I, RCC1
Positive Sample:
HeLa, Raji, SH-SY5Y, Mouse spleen, Mouse thymus, Rat testis
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
45kDa
Observed MW:
45kDa
Enables several functions, including guanyl-nucleotide exchange factor activity; nucleosomal DNA binding activity; and protein heterodimerization activity. Involved in several processes, including G1/S transition of mitotic cell cycle; regulation of mitotic nuclear division; and spindle organization. Located in chromatin; cytoplasm; and nucleus. Part of protein-containing complex.
Purification Method
Affinity purification
Gene ID
1104
RRID
AB_2861474
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RCC1 Rabbit mAb (CAB0662) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using RCC1 Rabbit mAb (CAB0662) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
