The CoREST/RCOR1 Antibody (CAB12845) is a high-quality antibody developed for reliable detection and analysis of target proteins. This cell surface molecule is involved in multiple cellular processes, including gene regulation and neurodevelopment. The antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in applications such as Western blotting.RCOR1 plays a critical role in epigenetic regulation and transcriptional repression, making it a key player in numerous biological processes. Its involvement in neural development and function also makes it a potential target for research in neurobiology and neurodegenerative diseases.
This antibody is validated for use in WB, IF/ICC, IP, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CoREST/RCOR1 Antibody
SKU:
CAB12845
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP
5μg antibody for 10μg-15μg of Chromatin
Synonyms:
RCOR, COREST, CoREST/RCOR1
Positive Sample:
HeLa, Jurkat, Mouse testis, Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
53kDa
Observed MW:
60kDa
This gene encodes a protein that is well-conserved, downregulated at birth, and with a specific role in determining neural cell differentiation. The encoded protein binds to the C-terminal domain of REST (repressor element-1 silencing transcription factor).
Purification Method
Affinity purification
Gene ID
23186
RRID
AB_2759686
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CoREST/RCOR1 Rabbit pAb (CAB12845) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg CoREST/RCOR1 antibody (CAB12845). Western blot was performed from the immunoprecipitate using CoREST/RCOR1 antibody (CAB12845) at a dilution of 1:1000.
