Description
Recombinant Human AGER/RAGE Protein
The Recombinant Human AGER/RAGE Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating AGER/RAGE function and its implications in health and disease.
This product (SKU: RPCB0249) is produced using HEK293 cells and features a C-hFc&His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 60.89 kDa with an observed molecular weight of 80-100 kDa under denaturing conditions, achieving ≥ 90% as determined by SDS-PAGE;≥ 90% as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human AGER/RAGE Protein |
| SKU: | RPCB0249 |
| Size: | 20 μg , 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | AGER, RAGE, SCARJ1 |
| Tag: | C-hFc&His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 60.89 kDa |
| Observed MW: | 80-100 kDa |
| Gene ID: | 177 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human AGER/RAGE Protein (RPCB0249), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 90% as determined by SDS-PAGE;≥ 90% as determined by HPLC. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Bio-Activity: |
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| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Receptor for Advanced Glycosylation End Products (RAGE, or AGER) is a member of the immunoglobulin super-family transmembrane proteins, as a signal transduction receptor which binds advanced glycation endproducts, certain members of the S100/calgranulin family of proteins, high mobility group box 1 (HMGB1), advanced oxidation protein products, and amyloid (beta-sheet fibrils). It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as atherosclerosis, arthritis, Alzheimer's disease, atherosclerosis and aging associated diseases.
 | Recombinant Human AGER/RAGE Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | The purity of Human AGER/RAGE Protein (RPCB0249) was greater than 90% as determined by SEC-HPLC. |
 | Immobilized Human S100A12 at 2 μg/mL (100 μL/well) can bind recombinant Human AGER/RAGE, the EC50 of Human AGER/RAGE is 27.25 ng/mL. |
