Recombinant Human ApolipoproteinA-IV/APOA4 Protein
The Recombinant Human ApolipoproteinA-IV/APOA4 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating ApolipoproteinA-IV/APOA4 function and its implications in health and disease.
This product (SKU: RPCB1223) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 44.22 kDa with an observed molecular weight of 45-55 kDa under denaturing conditions, achieving ≥ 90% as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human ApolipoproteinA-IV/APOA4 Protein
High quality, high purity and low endotoxin recombinant Recombinant Human ApolipoproteinA-IV/APOA4 Protein (RPCB1223), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 90% as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Apolipoprotein A-IV (ApoA-IV), first discovered in rat plasma in the 1970s, is a 46 kD glycoprotein with relatively high concentrations in plasma (15–37 mg/dL). It is expressed predominantly in the mammalian small intestine (with highest expression in the duodenum), and also expressed in rodent liver at lower levels. Sensing lipid absorption in enterocytes, ApoA-IV is synthesized and packaged with lipids and other apolipoproteins into chylomicrons and secreted through the lymphatic system into the blood. Once there, as a member of exchangeable apolipoproteins, ApoA-IV dissociates from chylomicrons and transfers to HDL (high density lipoprotein) particles or circulates in lipid-free form.ApoA-IV may protect against atherosclerosis, mediate reverse cholesterol transport, act as an anti-inflammatory agent and modulate intestinal lipid absorption.
Recombinant Human ApolipoproteinA-IV/APOA4 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
