Recombinant Human Atrophin-1 interacting protein 4/ITCH protein
The Recombinant Human Atrophin-1 interacting protein 4/ITCH protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Atrophin-1 interacting protein 4/ITCH protein function and its implications in health and disease.
This product (SKU: RPCB2059) is produced using E. coli and features a NO-Tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 102.67 KDa with an observed molecular weight of 100-140 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Atrophin-1 interacting protein 4/ITCH protein
High quality, high purity and low endotoxin recombinant Recombinant Human Atrophin-1 interacting protein 4/ITCH protein (RP10137LQ), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
Please contact us for more information.
Purity:
≥ 90 % as determined by SDS-PAGE.
Formulation:
0.22 μm filtered solution of PBS, pH 7.4.
Storage:
Store at -70°C. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
ITCH, also called atrophin-1 interacting protein 4 (AIP4), is a HECT-type E3 ubiquitin ligase. It contains an N-terminal Ca -dependent phospholipid-binding C2 domain, four WW domains, and a C-terminal HECT domain. Mice deficient in ITCH protein display many autoimmune-like disease characteristics such as lymphoproliferation in the spleen and lymph nodes. More than a dozen of substrates of ITCH have been identified including p63, p73 and Notch1. The ligase may be responsible for ubiquitinating proteins with K29-, K48-, and/or K63-linked ubiquitin chains.
Recombinant Human Atrophin-1 interacting protein 4/ITCH protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
