The Recombinant Human BMPR-1B/ALK-6/CDw293 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating BMPR-1B/ALK-6/CDw293 function and its implications in health and disease.
This product (SKU: RPCB1317) is produced using HEK293 cells and features a C-hFc&His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 39.50 kDa with an observed molecular weight of 50-55 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human BMPR-1B/ALK-6/CDw293 Protein
SKU:
RPCB1317
Size:
10 μg , 20 μg , 50 μg , 100 μg
Reactivity:
Human
Synonyms:
ALK-6, ALK6, AMDD, BDA1D, BDA2, CDw293, BMPR1B, ALK-6, ALK6, AMDD, BDA1D, BDA2, CDw293, bone morphogenetic protein receptor type-1B
High quality, high purity and low endotoxin recombinant Recombinant Human BMPR-1B/ALK-6/CDw293 Protein (RPCB1317), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 90 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human BMP2 at 4 μg/mL (100 μL/well) can bind Human BMPRIB with a linear range of 20-338 ng/mL.
Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED 50 for this effect is 0.6-2.3 μg/mL in the presence of 30 ng/mL of rhBMP-4.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant Human BMPR-1B/ALK-6/CDw293 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human BMP2 at 4 μg/mL (100 μL/well) can bind Human BMPRIB with a linear range of 20-338ng/mL.
