Cathepsin S is a lysosomal enzyme that belongs to the papain family of cysteine proteases. This protein is expressed by antigen presenting cells including macrophages, B-lymphocytes, dendritic cells and microglia. Moreover, cathepsin S is expressed in some epithelial cells. Compared with the abundant cathepsins B, LandH, cathepsin S shows a restricted tissue distribution, with highest levels in spleen, heart, and lung. In addition, evidences indicated that cathepsin S generates A beta from amyloidogenic fragments of beta APP in the endosomal/lysosomal compartment, and is implicated in the pathogenesis of AlzheimerÂ’s disease(AD) and Down Syndrome (DS).
Product Name:
Human Cathepsin S/CTSS Recombinant Protein (RPES3899)
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 The specific activity is 9063.642 pmol/min/µg.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Cathepsin S is produced by our Mammalian expression system and the target geneencoding Gln17-Ile331(pro)&Ser109-Ile331&Gln17-Ser109(Propeptide) is expressed with a 6His tag atthe C-terminus.
Purity:
> 90% as determined by reducing SDS-PAGE.
Mol Mass:
36.9&25&11.2 kDa
AP Mol Mass:
37-40&26-28&14-16 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM MES, 150mM NaCl, 10% Glycerol, pH 5.5.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
