CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein from the TNF superfamily. CD40LG exists as a trimer in membrane-bound and soluble form, both of which are bioactive. CD40L is a CD40 ligand that activates signal transduction in CD40LG-expressing cells through ligation. CD40LG is a differentiation antigen that T-cells express on their surface. It stimulates B-cell proliferation and production of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been found to generate and destroy tumour cells in peripheral blood monocytes. It also co-stimulates the proliferation of activated T-cells and this is accompanied by the production of IFN-gamma; TNF-alpha; and IL2. Human CD40L/TNFSF5 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Immobilized Human CD40L at 10µg/ml(100 ?l/well) can bind Human CD40-His(Cat: PKSH033723).
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human CD40 Ligand is produced by our Mammalian expression system and the target gene encoding Met113-Leu261 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
16.2 kDa
AP Mol Mass:
16-18 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 200mM NaCl; 0.1mM EDTA; pH 7.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
