The Recombinant Human CD47 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating CD47 function and its implications in health and disease.
This product (SKU: RPCB1526) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 14.56 kDa with an observed molecular weight of 35-45 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human CD47 Protein (RPCB1526), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 5 μg/mL (100 μL/well) can bind Human SIRPA with a linear range of 0.488-189.26 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human CD47 (RPCB1526) at 2 μg/mL (100 μL/well) can bind Human SIRP-alpha/CD172a (RPCB0297) with a linear range of 0.06-22.3 ng/mL. 3.Loaded Human SIRP-alpha/CD172a Protein, C-hFc&His(RPCB0297) on ProA Biosensor, can bind Human CD47 Protein, C-His Tag (RPCB1526) with an affinity constant of 11.1 nM as determined in BLI assay (Gator).
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
This protein is a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes.
Recombinant Human CD47 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human CD47 at 5 μg/mL (100 μL/well) can bind Human SIRPA with a linear range of 0.488-189.26 ng/mL.
Loaded Human SIRP-alpha/CD172a Protein, C-hFc&His(RPCB0297) on ProA Biosensor, can bind Human CD47 Protein, C-His Tag (RPCB1526) with an affinity constant of 11.1 nM as determined in BLI assay (Gator).
