Neural cell adhesion molecule L1-like protein, also known as close homolog of L1 (CHL1) is the prototypic member of the CTF / NF-1 family of transcription factors that serve as a novel calcium signaling pathway-responsive transcription factor and is considered as a member of the largest ctf complementation group, consisting of 30 of 126 ctf mutants isolated. CHL1 is a cell adhesion molecule highly related to L1. It contains structure plan of six extracellular C2-type immunoglobulin (Ig) domains followed by five fibronectin type? domains linked by a single membrane-spanning region to a short cytoplasmic domain. The extracellular portion of CHL1 is higyly glycosylated and involved them in hemophilic disease.
Product Name:
Human CHL Recombinant Protein (RPES1460)
Product Code:
RPES1460
Size:
50 µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CALL,L1CAM2,LICAM2
Accession:
AAI04919.1
Sequence:
Met 1-Gln 1080
Fusion tag:
C-His
Activity:
Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to CHL1 coated plates (0.8 µg/ml and 100 ?l/well), approximately 40%-60% will adhere specifically after 60 minutes at 37?.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of human CHL1 (AAI04919.1) (Met 1-Gln 1080) was fused with a polyhistidine tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
120 kDa
AP Mol Mass:
160-180 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
