COL9A1; which is short for Collagen alpha-1(IX) chain; is a 921 aa. protein. It is a secreted protein; and exists in extracellular space and extracellular matrix. This protein is a heterotrimer of an alpha 1(IX); an alpha 2(IX) and an alpha 3(IX) chain.Each subunit is composed of three triple-helical domains interspersed with non-collagenous domains. The globular domain at the N-terminus of type IX collagen molecules represents the NC4 domain which may participate in electrostatic interactions with polyanionic glycosaminoglycans in cartilage. It ia a structural component of hyaline cartilage and vitreous of the eye.
Recombinant Human Collagen alpha-1(IX) chain is produced by our Mammalian expression system and the target gene encoding Ala24-Pro328 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
33.8 kDa
AP Mol Mass:
38-50 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl; pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
