Epidermal growth factor (EGF) is a little 53 amino acid protein with three disulfide connections. It's a mitogenic protein that's been linked to cell development, tumour formation, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein has both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which competes for EGF receptor sites. Human EGF Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn971-Arg1023 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
6.2 kDa
AP Mol Mass:
11 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris, 200mM NaCl, pH 8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
