Description
Recombinant Human Fc gamma RI/FCGR1/CD64 Protein
The Recombinant Human Fc gamma RI/FCGR1/CD64 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Fc gamma RI/FCGR1/CD64 function and its implications in health and disease.
This product (SKU: RPCB1509) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 33.23 kDa with an observed molecular weight of 45-60 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human Fc gamma RI/FCGR1/CD64 Protein |
| SKU: | RPCB1509 |
| Size: | 10 μg , 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | FCGR1A, CD64, CD64A, FCRI, IGFR1, Fc fragment of IgG receptor Ia, CD64, CD64A, FCRI, IGFR1 |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 33.23 kDa |
| Observed MW: | 45-60 kDa |
| Gene ID: | 2209 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Fc gamma RI/FCGR1/CD64 Protein (RPCB1509), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Bio-Activity: | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human FCGR1A, His Tag at 2 μg/mL (100 μL/well) can bind Rituximab with a linear range of 15.625-58.38 ng/mL. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation. The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities only recognizing IgG aggregates surrounding multivalent antigens. Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta. The only inhibitory member in human and mouse, Fc gamma RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses. Three highly homologous genes (A, B, and C) sharing 98% identity at the nucleotide level have been identified for the human CD64 group. Fcgamma RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associated Fc R gamma accessory chain. The genes for Fc gamma RIB and Fc gamma RIC contain stop codons within their membrane proximal Ig-like domains indicating possible secreted receptors.
 | Recombinant Human Fc gamma RI/FCGR1/CD64 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | The purity of human Fc gamma RI/CD64 Protein (RPCB1509) was greater than 90% as determined by SEC-HPLC. |
